培養基及成分
1. Acetobacter Medium (醋酸菌培養基)
Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g
CaCO3 20g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml Adjust (調) pH to 6.8
適用範圍︰惡臭醋酸桿菌混濁變種
2. Nutrient Agar (營養肉汁瓊脂)
Pepton (蛋白 ) 5g Beef extract (牛肉膏) 30g
NaCl 5g Agar (瓊脂) 15g
Distilled water (蒸餾水) Adjust (調) pH to 7.0-7.2
[Note]︰When cultivation of Bacillus,5mg of to MnSO4.H2O may be added .
It is favorable to promote spore formation .
適 用範圍︰產氣氣桿菌、糞產堿桿菌、蠟狀芽孢桿菌、蠟狀芽孢桿菌蕈狀變種、地衣形芽孢桿菌、巨大芽孢桿菌、多粘芽孢桿菌、塵埃芽孢桿菌、短小芽孢桿菌、嗜熱 脂肪芽孢桿菌、炫顗痼U桿菌、炫顗痼U桿菌深黑變種、甦雲金芽孢桿菌、甦雲金芽孢桿菌蠟螟亞種(青蟲菌)、甦雲金芽孢桿菌戈爾斯德變種、甦雲金芽孢桿菌猝 倒亞種、產氨短桿菌、黃色短桿菌、谷氨酸棒狀桿菌、北京棒桿菌、大腸埃希氏菌(大腸桿菌)、銅綠假單胞菌(綠膿桿菌)、凸形假單胞桿菌、熒光假單胞菌、彎 曲假單胞菌、惡臭假單胞菌、假單胞桿菌、藤黃八疊球菌、亞黃八疊球菌、尿素八疊球菌、金黃色葡萄球菌、運動發酵單孢菌
3. Azotobacter Medium (固氮菌培養基)
KH2PO4 0.2g K2HPO4 0.8g
MgSO4.7H2O 0.2g CaSO4.2H2O   0.1g
   Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g
Mannitol(甘露醇) 20g FeCl3 Tract(微量)
Distilled water (蒸餾水) 1000ml Agar (瓊脂) 15g
Adjust (調) pH to 7.2
適用範圍︰固氮菌、膠質芽孢桿菌
4. Corn Meal Medium (玉米粉培養基)
Maize flour (玉米粉) 5g Peptone (蛋白 ) 0.1g
Glucose (葡萄糖) 1g Tap water (自來水) 1000ml
[Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18 18 mm tubes , each contains 10 ml of the liquid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小時,分裝入18 18毫米試管,每管深度達6厘米。15磅再次滅菌15小時。)
5. Lactic-bacteria Medium I (乳酸菌培養基 I )
Yeast extract (酵母膏) 7.5g Peptone (蛋白 ) 7.5g
Glucose (葡萄糖) 10g KH2PO4 2g
Tomato juice (西紅柿汁) 100ml Tween (吐溫) 80 0.5ml
Distilled water (蒸餾水) 900ml pH 7.0
適用範圍︰植物乳桿菌(胚芽乳桿菌)、嗜熱乳酸鏈球菌
6. Lactic-bacteria Midium Ⅱ (乳酸菌培養基 Ⅱ)
Lacto-casein peptone (乳酪蛋白 ) 10g Beef extract (蛋白 ) 10g
Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g
Tween (吐溫) 80 1g K2HPO4 2g
Na-acetate (醋酸鈉) 5g Diamine citrate (檸檬酸二胺) 2g
MgSO4.7H2O 0.2g MnSO4.H2O 0.05g
Distilled water (蒸餾水) 1000m pH 6.5-6.8
適用範圍︰植物乳桿菌(胚芽乳桿菌)
7. Peotone Glucose Yeast extract Medium PGY (蛋白 、酵母膏、葡萄糖培養基)
Peptone(蛋白 ) 10g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 1g Distilled water (蒸餾水) 1L
8. Glycerol Agar (甘油瓊脂)
Peptone (蛋白 ) 5g Beef extract (酵母膏) 3g
Glycerol (甘油) 20g Top water (自來水) 1000ml
Agar (瓊脂) 15g pH 7.0-7.2
9. Rhizobium medium (根瘤菌培養基)AS 9
Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml
Mannitol (甘露醇) 10g Agar (瓊脂) 15g
Distilled water (蒸餾水) 800ml pH 7.2
[Note]︰Soil extract︰Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients and distributed. (土壤浸提液的制法︰取土壤50克,加水200毫升,15磅蒸煮1小時,經濾紙過濾後加水補足到200毫升。)
適用範圍︰大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫雲英根瘤菌、
大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌
10. Mannitol Agar (甘露醇瓊脂)
Yeast extract (酵母膏) 5g Peptone (蛋白 ) 3g
Mannitol (甘露醇) 25g Agar (瓊脂) 15g
Distilled water (蒸餾水) 1000ml


11. Glucose Asparagine (葡萄糖、天門冬素瓊脂)
Glucose (葡萄糖) 10g Asparagine (天門冬素) 0.5g
K2HPO4 0.5g Water (水) 1000ml
pH 7.2-7.4
適用範圍︰刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)
12. Gause′s Synthetic Agar (高氏合成一號瓊脂)
KNO3 1g Soluble starch(可溶性澱粉) 20g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4 0.01g
Agar (瓊脂) 20g water (水) 1000ml
pH 7.2-7.4
適 用範圍︰刺孢小單孢菌絳紅變種、紫色小單孢菌(絳紅小單孢菌)、白黃鏈霉菌、白色鏈霉菌、抗生鏈霉菌、雙重輪絲鏈霉菌、產色鏈霉菌、燼灰鏈霉菌、天藍色鏈 霉菌、滅蚊鏈霉菌、紅霉素鏈霉菌、青色鏈霉菌、球孢鏈霉菌、淺灰鏈霉菌、灰色鏈霉菌、吸水鏈霉菌、淡紫灰鏈霉菌、黃色長孢鏈霉菌、藤黃色鏈霉菌、細黃鏈霉 菌、黑化鏈霉菌、玫瑰色鏈霉菌、華美鏈霉菌、嗜熱鏈霉菌、委內瑞拉鏈霉菌、紫色直絲鏈霉菌、紫色鏈霉菌、綠色鏈霉菌
13. Wort Agar (麥芽汁瓊脂)
Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then distribute the medium into tubes. Autoclave at 110 for 30 minutes. (將發酵啤酒的原料(未加酒花),稀釋至12柏林,加瓊脂15克,溶化後分裝。15磅滅菌30分鐘。)

適用範圍︰克魯斯假絲酵母、郎比可假絲酵母、解脂假絲酵母、馬其頓假絲酵母、擬熱帶假絲酵母、粗壯假絲酵母、皺褶假絲酵母、熱帶假絲酵母、產朊假絲酵母、 阿舒假囊酵母、白地霉、果香地霉、地霉屬、異常漢遜酵母、異常漢遜酵母變種、阿拉伯糖醇漢遜酵母、施氏漢遜酵母、菅囊酵母、伯頓畢赤酵母、膜醭畢赤酵母、 粘紅酵母、小紅酵母、膠紅酵母、深紅酵母、卡爾斯伯酵母、釀酒酵母、椰園釀酒酵母、發酵性酵母、洛格酵母、羅斯酵母、魯氏酵母多形魯氏酵母、威爾酵母、酵 母、路德類酵母、栗酒裂殖酵母、擲抱酵母、貝雷絲孢酵母、皮狀絲孢酵母、糙孢曲霉、無殼曲霉、鮮橙曲霉、紅曲霉、紫紅曲霉、紅色紅曲霉、紅曲霉菌
14. Potato Dextrose Agar PDA (馬鈴薯、葡萄糖瓊脂)
Potato extract (馬鈴薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g
Agar (瓊脂) 20g
[Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until soft. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.(注︰取去皮馬鈴薯200克,切成小塊,加水1000毫升煮沸30分鐘,濾去馬鈴薯塊,將濾液補足至1000毫升,加葡萄糖20克,瓊脂 15克,溶化後分裝,15磅滅菌30分鐘。)
適用範圍︰酵米面假單胞菌(酵米面黃桿菌)、白色鏈霉菌、燼灰鏈霉菌、青色鏈霉
菌、球孢鏈霉菌、灰色鏈霉菌、龜裂鏈霉菌、傘枝梨頭霉、雅致放射毛霉、棒曲霉、
米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛殼、長刺毛殼、橄欖包
毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、束狀刺盤孢、新月彎孢霉、奇異翅孢殼、地
生翅孢殼、串珠鐮孢、尖鐮孢、盤長孢菌(魯保一號)、銀白楊盤長孢、玉蜀黍長蠕
孢、深黃被孢霉、小被孢霉、多頭被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷
枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀
毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬
青霉、擬青霉、球形阜孢、白腐菌、少根根霉、華根霉、科恩根霉、戴爾根霉、日根霉、爪哇根霉、米根霉、點頭根霉、綠霉、簇孢匍柄霉、雅致枝霉、康寧木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢
15. Czapek′s Agar(察氏瓊脂)
Sucrose (蔗糖) 30g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g K2HPO4 1g
Agar (瓊脂) 13g Distilled water (蒸餾水) 1000ml
pH 7.2
適 用範圍︰紅霉素鏈霉菌、洋蔥曲霉、金頭曲霉、泡盛曲霉、泡盛曲霉煙色變種、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、無花果曲霉、黃柄曲霉、黃曲霉、煙曲 霉、構巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、醬油曲霉、土曲霉、宇佐美曲霉、焦曲霉、雜色曲霉、溫特曲霉、阿達青霉、產黃青霉、黃綠青霉、桔青霉、 頂青霉、園弧青霉、繩狀青霉、灰黃青霉、島青霉、淡紫青霉、特異青霉、赭綠青霉、產紫青霉、婁地青霉、小刺青霉、蕁麻青霉、
16. Concentrated Sucrose Czapek′s (濃糖察氏瓊脂)
Sucrose (蔗糖) 200g NaNO3 3g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.4H2O 0.01g K2HPO4 1g
Agar (瓊脂) 13g Distilled water (蒸餾水) 1000ml
適用範圍︰謝瓦曲霉
17. Synthetic Potato Medium (綜合馬鈴薯培養基)
20%Potato extract (馬鈴薯汁) 1L Glucose (葡萄糖) 20g
KH2PO4 3g MgSO4.7H2O 1.5g
Vitanib B1 (硫胺素) Trace (微量) Agar (瓊脂) 15g
pH 6
適 用範圍︰米曲霉、醬油曲霉、棲土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、頂頭孢霉、巴西毛客、長刺毛殼、 球毛殼、橄欖包毛殼、反曲毛殼、琥珀毛殼、圓酵毛殼、多主枝孢、束狀刺盤孢、葫蘆科刺盤孢、新月彎孢霉、奇異翅孢殼、地生翅孢殼、串珠鐮孢、蝕脈鐮孢霉、 盤長孢菌(魯保一號)、銀白楊盤長孢、瓶毛殼、金龜子綠僵菌、生香毛霉、卷枝毛霉、兩型孢毛霉、直立毛霉、球孢毛霉、絲球毛霉、大毛霉、多型孢毛霉、總狀 毛霉、魯氏毛霉、五通橋毛霉、黑球漆斑菌、露濕漆斑菌、玫煙色擬青霉、棉鈴蟲擬青霉、淡紫色擬青霉、擬青霉、白腐菌、少根根霉、根霉屬的菌、華根霉、科恩 根霉、戴爾根霉、台灣根霉、日根霉、爪哇根霉、黑根霉、雪白根霉、少根根霉、米根霉、點頭根霉、三寶壟根霉、根霉屬的種、綠霉、康寧木霉、木素木霉、擬康 氏木霉、木霉、粉紅木霉、綠色木霉、黃萎輪枝孢、大麗花輪枝孢、雙孢蘑菇、大肥菇、發光假蜜環菌、木耳、皺木耳、毛木耳、大禿馬勃、長根菇、金針菇、棘托 竹蓀、短裙竹蓀、短裙竹蓀東北變種(暫定)、長裙竹蓀、白鬼筆、無裙竹蓀、寬鱗大孔菌、凸圓靈芝(白芝)、紫芝、靈芝(紅芝,赤芝,日靈芝,韓國靈芝)、 灰樹花、猴頭菌、香菇、粗毛斗菇、潔麗香菇、陞笸ㄐB玉蕈、真姬菇、安絡小皮傘菌、鬼毛針、黑柄皮傘菌、尖頂羊肚菌、圓錐羊肚菌、高羊肚菌、黑脈羊肚菌、 羊肚菌、滑菇、光帽黃傘、尖鱗黃傘、金頂妛e 芑頗ァ 鴝ツァ 諄榷 湘咂焦健 Ч健 啞榷 餑姨榷  愎劍  嫦榷替弒渲幀 嫦榷⑸刃紋焦健 廡紋焦健  鷳蘩鉲鏤捩咦榷 鷳蘩鉲榷Ⅱ汗健 諂榷⑵焦健 蔥榷 コ峋 渦榷  ⑹魑選 獗榷 牢榷 湘榷  榷 酆榷 鏤補健 ┐紛榷 芨榷 笥苣ァ榷④蜍摺 詮 健 躺 旃健 瘍蘧 ψ蕁 鴝 頗徑  啄徑 暇Э諛ァ 抗健⑺扛切“毆健 莨
18. Corn Meal Medium (玉米粉培養基)
Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉裝瓶,15磅滅菌30分鐘。)
19. Filter Paper Medium (濾紙培養基)
(NH4)2SO4 1g KH2PO4 1g
MgSO4.7H2O 0.7g NaCl 0.5g
Distilled water (蒸餾水) 1000ml A strip of filter paper (濾紙條) 6 1cm (厘米)
20. Soybean Cake Meal Agar (黃豆餅粉瓊脂)
10% infusion of soybean cake meal (10%黃豆餅粉浸汁) 1000ml

Glucose (葡萄糖) 10g NaCl 2.5g
[next]21. Sawdust, Wheat-bran Medium (木屑、麩皮培養基)
Sawdust of broad leaf tree 75g Wheat bran 25g
Preparation︰Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth .
殼斗科植物或闊葉樹的木屑 75克   麩皮 25克
制法︰加水適量充分混勻,水量以手捏時指間有水珠為宜,裝瓶並壓緊,在中心處扎一小洞,堵上棉塞,20磅滅菌1小時。
22. Pine Block or Pine Sowdust Medium (松木條或松木屑培養基)
1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (將10 2 1厘米的松木條浸泡在1-2%的蔗糖水中。使木條吸足糖水,裝瓶不宜過緊,用紙包好,20磅滅菌1小時。)
2. Pine Sawdust Medium
Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24%
Socrose (蔗糖) 1-2% Water (appropriate)水 (適量)
Autoclave at 20 lbs for 1 hr.(20磅滅菌1小時)
23. Rice Straw. Dung Medium (稻草、馬糞培養基)
Dried horse dung (or cow dung) (干馬糞或牛糞) 65%
Died straw (干稻草) 35% Urea (尿素) 0.5%
Calcium superhosphate (過磷酸鈣)0.7% Gypsum powder(石膏粉) 1%
Ammonium sulphate (硫酸銨) 0.3%
[Note]: Add appropriate water, pile them up and cause them fermentation. (水適量進行堆積發酵)
24. Straw Rice Bran Medium (稻草、米糠培養基)
Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25%
[Note]︰Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]︰選無霉稻草,切成長約3厘米的小段,用清水浸泡12小時,使充分吸水,然後傾去水,加米糠和水,水量以用手捏時指間有水珠為宜。)
25. Rice Medium (米飯培養基)
Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15 150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克,入50毫升椎形瓶中,水20-30毫升于15磅滅菌30分鐘即成,或取大米2克,裝入15 150毫米試管中,加水6毫升,于15磅滅菌 30分鐘。)

26. Filter Paper Medium (濾紙條培養基)
(NH4)2SO4 1g KH2PO4 1g
   MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g
Distilled Water (蒸餾水) 1000ml
Strip of filter paper 1 7 cm (濾紙條 1 7 厘米)
[Note]︰Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (將上述溶液分配成溶液後分裝入試管,每管1毫升,15磅滅菌30分鐘。再將滅菌的濾紙條(1條或2條)投入試管中即好使用。)
27. Yeast Extract-Medium Extract Agar I (酵母膏、麥芽膏瓊脂)
Yeast extract (酵母膏) 10g Mall extract (麥芽膏) 10g
Glucose (葡萄糖) 4g Distilled water (蒸餾水) 1000ml
Agar (瓊脂) 20g pH 7.0
適用範圍︰紅霉素鏈霉菌
28. Yeast Extract Peptone (酵母膏、蛋白 瓊脂)
Yeast extract (酵母膏) 1g Multi-peptone(多蛋白 ) 2g
Beef extract (牛肉膏) 1g Glucose (葡萄糖) 10g
Agar (瓊脂) 20g Distilled water (蒸餾水) 1L
pH 7.0
29. Glucose Yeast Extract Starch Agar (葡萄糖、酵母、澱粉瓊脂)
Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Starch (澱粉) 10g NaCl 5g
CaCO3 3g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1L pH 7.0
30. Wheat Bran Medium (麩皮培養基)
Wheat bran (麩皮) 36g (NH4)2HPO4 10g
K2HPO4 0.2g MgSO4。7H2O 0.1g
Agar (瓊脂) 20g Distilled water (蒸餾水) 1L
pH 7.0
31. Yeast Sporulation Medium (酵母菌產生子囊孢子培養基)
(1) Kleyn Medium
KH2PO4 0.012% K2HPO4 0.02%
Sodium acetate (醋酸鈉) 0.5% Glucose (葡萄糖) 0.062%
NaCl 0.062% Peptone (蛋白 ) 0.25%
Biotin (生物 Living beings素) 2μg% Agar (瓊脂) 2%
Trace element solution (混合鹽類) 1ml/100ml
[Note]: Trace element solution (混合鹽類溶液)︰ MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2%
(2) Gorodkowa Medium
Glucose (葡萄糖) 0.1% Peptone (蛋白 ) 1%
NaCl 0.5% Agar (瓊脂) 2%
(3) McClary Medium
Glucose (葡萄糖) 0.1% KCl 0.18%
Yeast juice (酵母汁) 0.25% Sodium actate (醋酸鈉) 0.82%
Agar (瓊脂) 1.5%
32. BPY Medium (BPY 培養基)
Beef extract (牛肉膏) 5g Prptone (蛋白 ) 10g
NaCl 5g Yeast extract (酵母膏) 5g
Glucose (葡萄糖) 5g Agar (瓊脂) 1-2%
Distilled (蒸餾水) 1000ml pH 7.0
適用範圍︰大腸埃希氏菌(大腸桿菌)、惡臭假單胞菌
33. LB Medium (LB 培養基)
Yeast extact (酵母膏) 5g Peptone (蛋白 ) 10g

NaCl 10g Agar (瓊脂) 1-2%
Distilled water (蒸餾水) 1000ml pH 7.0
適用範圍︰大腸埃希氏菌(大腸桿菌)
34. Penssay Broth (Penssay 肉湯)
Beef extract (牛肉膏) 1.5g Yeast extract (酵母膏) 1.5g
Tryptone (胰蛋白 ) 5g NaCl 3.5g
Glucose (葡萄糖) 1g K2HPO4.3H2O 4.8g
Distilled water (蒸餾水) 1000ml pH 7.2
35. Blond Agar (血瓊脂培養基)
Peptone (蛋白 ) 10g Glucose (葡萄糖) 2g
NaCl 5g NaH2PO4 2.5g
Sodium citrate (檸檬酸鈉) 5g Brain infusion (腦浸汁) 800ml
Pig heart infusion (豬心浸) 200ml Sleep Blood (羊血) 10%
pH 7.2
[Note]︰Preparation of pig brain and pig heart infusion︰
(1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml;
(2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml;
(3) Keep the two infusions at 50℃ for 1 hr , then boil for 15 minutes;
(4) Cool and filtrate through gauze . [注]豬心和豬腦浸汁法︰(1)新鮮豬心除去心頭和脂肪,攪碎100克加蒸餾水至1000毫升;(2)新鮮豬腦除去血管,取200克加蒸餾水至1000毫 升;(3)50℃保溫1小時,然後煮開15分鐘;(4)冷卻後用紗布過濾備用。

36. Cellulosedecomposing Bacteria Synthetic Medium (縴維細菌合成培養基)
NaCl 6g MgSO4.7H2O 0.1g
KH2PO4 0.5g CaCl2 0.1g
K2HPO4 2.0g Yeast extract (酵母膏) 1.0g
(NH4)2SO4 2.0g Distilled water (蒸餾水) 1000ml
Filter paper strip: one strip (濾紙條︰一片) pH 7.0-7.4
[Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (斜面培養基去濾紙條用2%瓊脂及2%羧甲基縴維素鈉 CMCNa )
37. Clostridium Pasreurianum Synthetic Medium (巴氏梭狀芽孢桿菌合成培養基)
Glucose (葡萄糖) 1% MnSO4 0.001%
KH2PO4 0.05% FeSO4 0.001%
K2HPO4 0.05% Yeast extract(酵母膏) 0.1%
MgSO4.7H2O 0.02% Peptone (蛋白 ) 0.01%
NaCl 0.001% CaCO3 0.5%
Distille water (蒸餾水) 100% pH 7.0
38. Yeast Extract-Medium Extract Agar Ⅱ (酵母膏、麥芽膏瓊脂 Ⅱ)
Yeast extract (酵母膏) 4g Mall extract (麥芽膏) 10g
Glucose (葡萄糖) 4g Agar (瓊脂) 17g
Distilled water (蒸餾) 1000ml pH 7.3
39. Oatemeal Agar (燕麥粉瓊脂)
Oatemeal(infusion)[燕麥粉浸液] 20g Agar (瓊脂) 18%
Trace element solution (跡量鹽溶液)1ml
Distilled water up to (用蒸餾水補充至1000毫升) 1000ml pH 7.2
[Note]︰Trace element solution (跡量鹽溶液)︰
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g Distilled water (蒸餾水) 100ml
40. Calcium Malate Agar (隻果酸鈣瓊脂)
Calium malate (隻果酸鈣) 10g K2HPO4 0.5g
NH4Cl 0.5g Glycerol (甘油) 10g
Agar (瓊脂) 15g Distilled water (蒸餾水) 1000ml
41. Thiobacillus Ferrooxidans Medium (氧化亞鐵硫桿菌培養基)
(NH4)2SO4 0.15g KH2PO4 0.05g
KCl 0.05g MgSO4.7H2O 0.5g
Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g
Distilled water (蒸餾水) 1000ml pH 2.0
42. Thiobacillus Thiooxidans Medium (氧化硫硫桿菌培養基)
(NH4)2SO4 0.3g KH2PO4 3-4g
CaCl2 (anhydrous) [無水] 0.25g MgSO4.7H2O 0.5g
FeSO4.7H2O 0.001g Distilled water (蒸餾水) 1000ml
pH 3.5-4
43. Plasma Substitute Medium (代血漿培養基)
Sucrose (蔗糖) 12.5-15% Peptone (蛋白 ) 0.5%
KH2PO4 0.03% Na2HPO4.12H2O 0.14%
pH 7.0-7.2
44. Skim Milk Medium (脫脂牛奶培養基) ATCC 183
脫脂牛奶 100g 去離子水 1000ml
適用範圍︰保加利亞乳桿菌、嗜熱乳酸鏈球菌
45. Pea Agar (豌豆瓊脂)
Glucose (葡萄糖) 1% Peptone (蛋白 ) 0.5%
NaCl 0.5% Agar (瓊脂) 2.0%
Pea infusion (counted by NH2) [豌豆浸汁(以NH2計)] 12-14 mg100
pH (before sterilization) [消前] 7.2-7.5
[Note]: Preparetion of pea infusion (豌豆浸汁的制法) :
Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (稱取白豌豆900克,置于5公升廣口瓶中,加自來水3公升,用硫酸調pH至4.3-4.5,再加氯仿30毫升(防腐),加膠塞並用紗布扎口(防止沖 脫),放37℃恆溫室7-10天。取出用濾紙過濾,濾液煮沸20分鐘,以除氯仿,並再次以濾紙過濾,除沉澱。濾液分裝,于120℃30分鐘滅菌。後冷藏備 用。)
The standard quality of pea infusion as following (豌豆浸汁質量標準)︰
Aminonitrogen(氨基酸): 160-200 mg100ml
Soluble phosphoruse(溶磷): 400-600 μgml
Color(色澤): auratus,transparent (金黃、透明)

46. Wheat Bran Medium (麥麩瓊脂)
Wheat bran (麥麩) 3.6% (NH4)2HPO4 0.3%
K2HPO4 0.02% MgSO4.7H2O 0.01%
Agar (瓊脂) 2% pH 7.0
適用範圍︰金霉素鏈霉菌、紅霉素鏈霉菌
47. Glucose Yeast Extract Agar (葡萄糖、酵母膏瓊脂)

Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g
Water (水) 1000ml Agar (瓊脂) 15g
pH 7.2
48. MMN Medium (MMN培養基)
CaCl2 0.05g MgSO4 0.15g
NaCl 0.025g FeCl3 (1%) 1.2ml
KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg
(NH4)2HPO4 0.25g Wort (12 Be′) 100ml
Glucose (葡萄糖) 10-15g Citric acid (檸檬酸) 0.2g
Distilled water (蒸餾水) 900ml pH 5.5 (左右)
適用範圍︰蜜環菌
49. Starch Agar (澱粉瓊脂)
Soluble starch (可溶性澱粉) 10g NaNO3 1g
MgCO3 1g K2HPO4 0.3g
NaCl 0.5g Agar (瓊脂) 20g
Distilled water (蒸餾水) 1000ml
50. 5 Be′Wort Agar (5 Be′麥芽汁瓊脂)
適用範圍︰園弧青霉、少根根霉、日本根霉、爪哇根霉
51. 5 Be′Koji Extract Agar (5 Be′米曲汁瓊脂)
適用範圍︰華根霉、三寶壟根霉
52. 發根農桿菌培養基(YEB)
Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L
Peptone (蛋白 ) 5g/L Sucrose (蔗糖) 5g/L
MgSO4.7H2O 0.4g/100ml Agar (瓊脂) 1.5g/100ml
pH 7.4
適用範圍:青為眾甇M菌
53. 青炸葥鷎i基 (Tm)
Peptone (蛋白 ) 10g Casein (干酪素水解物產) 1g
Glucose (葡萄糖) 5g Ddhro (純水) 1000ml
Agar(瓊脂) 15g pH 7.0
54. YM (Yeast Malt Agar)
酵母膏 3.0g 麥芽汁 3.0g
蛋白  5.0g 瓊脂 20.0g
蒸餾水 1000ml
55. ISP 4
可溶性澱粉 10.0g K2HPO4 1.0g
MgSO4.7H2O 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
瓊脂 20.0g 蒸餾水 1000ml

56. Trypton Soy Agar
胰蛋白  17.0g 大豆  3.0g
葡萄糖 2.5g 瓊脂 20.0g
NaCl 5.0g K2HPO4 2.5g
蒸餾水 1000ml
57. NA (Nutrient Agar)
牛肉膏 3.0g 蛋白  5.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 6.6-7.0
58. 甘露(糖)醇瓊脂 (ATCC 1)
酵母膏 5.0g 蛋白  3.0g
甘露(糖)醇 25.0g 瓊脂 15.0g
蒸餾水 1000ml
59. Marine瓊脂 2216 (ATCC 2)
蛋白  5.0g 酵母膏 1.0g
檸檬酸鐵 0.1g 溴化鉀 0.08g
NaCl 19.45g MgCl2 8.8g
Na2SO4 3.24g CaCl2 1.8g
KCl 0.55g NaHCO3 0.16g
NH4NO3 0.0016g Na2HPO4 0.008g
氯化鍶 0.034g 硼酸 0.022g
 酸鈉 0.004g 氟化鈉 0.0024g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.4-7.8
60. 兔血瓊脂(ATCC 4)
牛心(取其浸液) 500.0g 胰蛋白  (Tryptose) 10.0g
NaCl 5.0g 瓊脂 15.0g
蒸餾水 1000ml pH 6.6-7.0
61. 孢子形成培養基 (ATCC 5)
酵母膏 1.0g 牛肉膏 1.0g
胰蛋白  2.0g 葡萄糖 10.0g
FeSO4 微量 瓊脂 15.0g
蒸餾水 1000ml pH 7.2
62. Trypticase Soy Agar (ATCC 18)
Trypticase Soy Broth 30.0g 瓊脂 15.0g
蒸餾水 1000ml
63. YPAD (ATCC 109)
酵母膏 10.0g 蛋白  20.0g
葡萄糖 20.0g 瓊脂 20.0g
蒸餾水 1000ml pH 7.0
64. SP (ATCC 432)
棉子糖 1.0g 蔗糖 1.0g
半乳糖 1g 可溶性澱粉 5g
水解酪蛋白 2.5g 瓊脂 20.0g
MgSO4.7H2O 0.5g K2HPO4 0.25g
蒸餾水 1000ml pH 自然
65. 胰酪 大豆酵母浸膏瓊脂 (TSA-YE)
胰蛋白  15.5g 大豆蛋白  5.0g
NaCl 5.0g 酵母膏 6.5g
瓊脂 15.0g 蒸餾水 1000ml
pH 7.3

66. ATCC 20
蛋白  5.0g 酵母膏 15.0g
K2HPO4 3.0g 葡萄糖 2.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.3-7.5
67. TY (Yeast Tryptone Agar)
蛋白  10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.0
68. 硫醇(Thiol)培養基 (ATCC 49)
示蛋白 (Proteose Peptone No.3,Difco) 10.0g 酵母膏 5.0g
葡萄糖 1.0g NaCl 5.0g
硫醇復合物 8.0g 瓊脂 1.0g
p-氨基苯甲酸 0.05g pH 6.9-7.3
69. Czapek’Dox 瓊脂培養基 (ATCC 134)
Czapek’Dox Broth 35.0g 瓊脂 15.0g
蒸餾水 1000ml
70. MG/L
蛋白  5.0g 甘露醇 5.0g
谷氨酸鈉 1.15g 生物 Living beings素 0.0001g
K2HPO4 0.25g NaCl 0.1g
MgSO4.7H2O 0.1g 酵母膏 2.5g
瓊脂 20.0g 蒸餾水 1000ml
氯霉素(終濃度) 50μg/ml
pH 7.0

71. Eclb Broth
蛋白  10.0g 酵母膏 5.0g
NaCl 5.0g 瓊脂 20.0g
蒸餾水 1000ml 氨芐青霉素(終濃度) 300μg/ml
pH 7.0
72. Bennetts Agar (IFO 228)
酵母膏 1.0g 牛肉膏 1.0g
NZ Amine,type A 2.0g 葡萄糖 10.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.3
73. Harrolds M40Y (ATCC 319)
麥芽膏 20.0g 酵母膏 5.0g
蔗糖 400.0g 瓊脂 20.0g
蒸餾水 1000ml
74. PSA (Potato Sucrose Agar)
土豆 200g 蔗糖 20g
蒸餾水 1000ml 瓊脂 20g
pH 6.0-6.5
75. 培養基
牛肉膏 300g 水解酪蛋白 17.5g
可溶性澱粉 1.5g 瓊脂 20.0g
蒸餾水 1000ml 磺胺嘧啶 (終濃度) 100μg/ml
氨芐青霉素 (終濃度) 100μg/ml pH 7.3-7.4

76. 馬鈴薯牛肉汁培養基
麥芽糖 20.0g 牛肉膏 5.0g
蛋白  3.0g 馬鈴薯 200.0g
pH 7.0-7.2
77. ISP 2
酵母膏 4.0g 麥芽汁 10.0g
葡萄糖 4.0g 瓊脂 20.0g
蒸餾水 1000ml pH 7.0
78. BHI (Brain Heart Infusion)
牛腦 200.0g 牛心浸出汁 250.0g
蛋白  10.0g 葡萄糖 2.0g
NaCl 5.0g 瓊脂 20.0g
蒸餾水 1000ml pH 6.8-7.2
79. YPG (Yeast Peptone Glucose)
酵母膏 10.0g 蛋白  20.0g
葡萄糖 20.0g 瓊脂 20.0g
蒸餾水 1000ml pH 7.0

80. 兔食瓊脂培養基(ATCC 340)
兔食(商店出售) 25.0g 瓊脂 20.0g
蒸餾水 1000ml
*煮沸兔食,並浸泡1/2小時,用多層紗布過濾,加瓊脂于濾液中。
81. 土豆瓊脂(ATCC 423)
200克去皮土豆切成小塊于1000ml自來水中煮沸1小時,用細棉布過濾.加進5.0mgMnSO4,pH調至6.8,用自來水定容至1000ml。加進15.0g瓊脂,溶化,分裝並滅菌。
82. 土豆浸汁加無機鹽培養基(ATCC 470)
土豆 2000g MnSO4.5H2O 0.02g
MgSO4.7H2O 0.4g ZnSO4.7H2O 0.01g
CuSO4. 5H2O 0.01g FeSO4.7H2O 0.1g
CaCl2.2H2O 0.1g K2HPO4 0.5g
* 稱取200g去皮塊狀土豆,加入1000ml蒸餾水煮沸30分鐘,多層紗布過濾,濾液中加入以上成分,溶解後定容至1000ml。

83. 無機鹽澱粉瓊脂培養基(ATCC 527)
可溶性澱粉 10.0g K2HPO4 1.0g
MgSO4 1.0g NaCl 1.0g
(NH4)2SO4 2.0g CaCO3 2.0g
FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g
ZnSO4.7H2O 0.001g 瓊脂 20.0g
pH 7.0-7.4
84. (AS125)
新鮮雞蛋 15-16 蒸餾水 375ml
2%孔雀(石)綠 10.0g K4HPO4 1.5g
MgSO4.7H2O 0.375g 天冬 胺 2.25g
甘油 7.5ml 土豆粉 18.8g
85. 燕麥片(Oatmeal)瓊脂培養基
燕麥片 60.0g 瓊脂 12.5g
蒸餾水 1000ml
* 將燕麥片加600ml水中,制成勻漿,加熱至45-50oC,然後加入已溶化瓊脂的另400ml水,121oC滅菌90分鐘。

86. 細菌培養基(ATCC 573)
(NH4)2SO4 1.3g K2HPO4 0.37g
MgSO4.7H2O 0.25g CaCl2.2H2O 0.07g
FeCl3 0.02g 葡萄糖 1.0g
酵母膏 1.0g 蒸餾水 1000ml
用10N H2SO4 調pH至4.0。若配制固體培養基,為增強上述溶液的強度,pH需調至3.5,瓊脂水溶液濃度為40g/L。兩者分別高壓滅菌,冷卻至約50oC,無菌操作將兩種溶液等體積混合,這一過程可避免瓊脂被酸水解。
87. 碎肉培養基(ATCC 593)
牛肉(去脂肪) 500.0g 蒸餾水 1000ml
NaOH 25.0ml
* 所用瘦牛肉或馬肉(碾)磨碎前需去脂肪和周圍的組織。將肉、水、NaOH混合後邊攪拌邊加熱,冷至室溫,去掉表面油脂,過濾,留下肉末和濾液。濾液加蒸餾水至1000ml。濾液中加下列成分︰
蛋白  30.0g 酵母膏 5.0g
K2HPO4 5.0g 0.025%刃天青(Resazurin)溶液4.0ml
然後煮沸,冷卻,加0.5g L-半胱氨酸HCl。H2O,pH調至7.0。在氧氣、氮氣(O2-freenitrogen)和3%氫氣條件下,按1份碎肉加4-5份以上溶液的比例分裝于每支試管。在氮氣和氫氣條件下,使用橡皮塞,快排氣,高壓滅菌15分鐘。
88. YMPG
酵母膏 3.0g 麥芽汁 3.0g
蛋白  5.0g 葡萄糖 10.0g
FeSO4.7H2O 0.1g 瓊脂 20.0g
蒸餾水 1000ml pH 6.8
滅菌︰110oC,35分鐘
89. TGYM培養基(ATCC 679)
胰蛋白  5.0g 葡萄糖 1.0g
酵母膏 3.0g 蒸餾水 1000ml
DL-蛋氨酸(DL-Meth 0.5g
90. TYG培養基(ATCC 741)
胰蛋白  3.0g 酵母膏 3.0g
葡萄糖 3.0g K2HPO4 1.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.4

91. M9
Na2HPO4 12.8g KH2PO4 3.0g
NaCl 0.5g NH4Cl 0.5g
葡萄糖(單獨滅菌) 10.0g 維生素B1(單獨滅菌) 1μg/ml
pH 自然
92. ISP3 (IFO 245)
燕麥粉 20.0g 微量元素溶液 1ml
pH 7.2
微量元素︰
FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g
ZnSO4.7H2O 0.1g
93. Oatmeal Agar
瓊脂 5.0g 蒸餾水 500ml
加熱溶化速溶燕麥粉 40.0g 蒸餾水 250ml
加熱溶化後與瓊脂液混合加水至1000ml pH 5.8
94. V8-Juice Agar (AYCC 343)
V-8 果汁 200g CaCO3 3.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 5.8
95. Czapek-Dox Agar (ATCC 312)
NaNO3 3.0g K2HPO4 1.0g
MgSO4.7H2O 0.5g KCl 0.5g
FeSO4.7H2O 0.01g 蔗糖(單獨滅菌) 30.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 5.6

96. GC培養基 (ATCC 814)
A 液
瓊脂 5.0g GC瓊脂培養基(BBL 11275) 36.0g
蒸餾水 500.0ml
B 液
干燥色牛血紅蛋白(BBL 11871) 10.0g 蒸餾水 500.0ml
Iso Vitale X (BBL 11876) 10.0ml
97. 固氮螺菌培養基 (ATCC 838)
K2HPO4 0.1g KH2PO4 0.4g
MgSO4.7H2O 0.2g NaCl 0.1g
CaCl2 0.02g FeCl3 0.01g
NaMoO4.2H2O 0.002g 隻果酸鈉(Na malate) 5.0g
酵母膏 0.05g 蒸餾水 1000ml
pH 7.2-7.4
98. BG-11
NaNO3 1.5g K2HPO4 0.04g
MgSO4.7H2O 0.075g CaCl2.7H2O 0.036g
Na2CO3 0.02g 檸檬酸 0.006g
檸檬酸鐵 0.006g *微量元素溶液 A5 1ml
氨芐青霉素 (終濃度) 50μg/ml 蒸餾水 1000ml
瓊脂 20.0g
* 微量元素溶液 A5
H3BO4 2.86g MnCl2.4H2O 1.81g
ZnSO4 0.222g Na2MoO4 0.39g
CuSO4.5H2O 0.079g Co(NO3)2.6H2O 49.4g
99. (Marine Agar)
蛋白  5.0g 酵母膏 1.0g
檸檬酸鐵 0.1g NaCl 19.45g
MgSO4.7H2O 5.9g KCl 0.55g
Na2SO4 3.24g CaCl2 1.8g
NaHCO3 0.16g KI 0.08g
微量元素 10ml pH 7.0-7.6
* 微量元素溶液︰
氯化鍶 0.034g  酸鈉 0.004g
蒸餾水 1000ml H3BO4 0.022g
NaCl 0.0024g NaNO3 0.0016g
NaHPO4 0.008g 蒸餾水 1000ml
100.(Yeast Starch Agar)
酵母膏 2.0g 可溶性澱粉 10.0g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.2-7.4

101.GYMC
葡萄糖 4.0g 酵母膏 2.0g
麥芽汁 20.0g 瓊脂 20.0g
CaCO3 1.5g 蒸餾水 1000ml
pH 6.8-7.0
102. LCSB
乳糖 15.0g 玉米漿 5.0g
蛋白  5.0g NaCl 4.0g
MgSO4.7H2O 0.5g KH2PO4 0.6g
FeCl3.6H2O 0.005g CuSO4.5H2O 0.002g
瓊脂 20.0g 蒸餾水 1000ml
相對濕度 65% pH 4.8
103.高氏1號(G)

可溶性澱粉 20.0g KNO3 1.0g
K2HPO4 0.5g MgSO4.7H2O 0.5g
NaCl 0.5g FeSO4.7H2O 0.01g
瓊脂 20.0g 蒸餾水 1000ml
pH 7.0-7.2
104.葡萄糖-酵母膏瓊脂(ATCC 846)
酵母膏 5.0g 蛋白  1.0g
KH2SO4 1.0g NaCl 1.0g
瓊脂 20.0g 蒸餾水 950.0ml
pH 6.8
補加50ml10%葡萄糖(葡萄糖溶液單獨過濾滅菌)。
105.Tep尿液(Uric Acid)瓊脂(ATCC 894)
Na2HPO4.12H2O 9.0g KH2PO4 1.5g
MgSO4.7H2O 0.2g 檸檬酸鐵銨(綠色) 1.2g
CaCl2 20.0mg MnCl2.4H2O 1.0mg
Trypticcase Soy Broth (BBL 11768) 3.0g 尿素 4.0g
瓊脂 20.0 蒸餾水 1000ml
pH 7.2
106.增強梭菌生長的培養基 (ATCC 1053)
胰蛋白  10.0g 牛肉膏 10.0g
酵母膏 3.0g 葡萄糖 5.0g
NaCl 5.0g 可溶性澱粉 1.0g
半胱氨酸鹽酸鹽 0.5g 醋酸鈉 3.0g
瓊脂 15.0g 蒸餾水 1000ml
pH 6.6-7.0
107.酵母膏-甘露(糖)醇瓊脂 (ATCC 1205)
K2HPO4 0.5g MgSO4.7H2O 0.2g
NaCl 0.1g 甘露(糖)醇 10.0g
酵母膏 0.4g 蒸餾水加至 1000ml
108.Tomato Dextrin Agar (ATCC 965)
番茄汁 20.0g 糊精 20.0g
酵母膏 10.0g CoCl2.6H2O 5.0mg
瓊脂 20.0g 蒸餾水 1000ml
pH 7.2-7.4
109.嗜熱菌無機鹽溶液 (ATCC 1554)
FeSO4 1.0mg MgSO4.7H2O 200.0mg
Na2HPO4 210.0mg NaH2PO4 90.0mg
KCl 40.0mg CaCl2 15.0mg
NaNO3 0.25g NH4Cl 0.25g
微量元素溶液(見下面) 10.0ml
n-Heptadecane(C17) 0.1%(v/v) 蒸餾水 1000ml
微量元素溶液︰
CuSO4.5H2O 500mcg H3BO4 1.0mg
MnSO4.5H2O 7.0mcg ZnSO4.7H2O 7.0mg
MoO3 1.0mg CoSO4.7H2O 18.0mcg
蒸餾水 1000ml
110.醋桿菌培養基 (AS 1)
葡萄糖 100.0g 酵母膏 10.0g
CaCO3 20.0g 瓊脂 15.0g
蒸餾水 1000ml pH 6.8



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